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KMID : 0352519940310010001
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Korea Univercity Medical Journal
1994 Volume.31 No. 1 p.1 ~ p.16
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Effect of Etretinate on Cell Proliferation in Limb Debvelopment
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Abstract
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Cell proliferation in the developmental sequence of fore-limb in the DDY mouse with the adverse effect of single dose of etretinate (Tigason(r)) on it correlated to the drug-induced dysmorphogenesis was studied by measns of
argyrophil technique for nucleolar organizer regions(AgNORs) and expression of proliferating cell nuclear antigen(PCNA).
The number of AgNORs per nucleus displayed regional differences in the limb bud on day 11 of gestation, as higher values in apical ectodermal ridge(AER) and growing tip but decreased rapidly thereafter compared with those in the other
parts
of
epithelia and subepithelial mesenchyme, while in core mesenchyme where the higest in its value on day 11, the numbers decreased depend not on the age but on the chondrified stage specific. Antibody reaction to PCNA expressed in
almost
all of the cells, stronger in mesenchyme than in epithelia on day 11 and then decreased gradually with the development proceed.
Siver reactions to NORs as well as PCNA expression were decreased sharply by etretinate but recovered gradually from 24 hours after treatment except the value of AgNORs in the chondrified mesenchyme.
The results indicate that growth conducted by AER with subridge mesenchyme and chondrification in the core mesenchyme are two of major processes in the early development of limb, and the adverse effects of etretinate on proliferation
and
differentiation persist longer in the chondrified area but decreased expression of proliferative activities by the drug do not appear to be the result subsequent to the drung-induced cell death.
The results suggest that temporal inhibition or interruption of the proliferation could deprive the programmed sequence of development of its appropriate timing with subsequent disturbance of the pace in growth which might be a
role
limb dysmorphogensis but the drug's affinity to the chondrified cells remains a question.
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